Fingerprint and Multivariate Analysis of Apium Graveolens L. From Different Geographic with Spectroscopic ATR-FTIR

Background: Apium graveolens L. or widely known as celery is a plant that comes from the Apiaceae family. Apium graveolens L. is found in the Indonesian Herbal Pharmacopoeia as a nutritious plant. Plants that spread throughout Indonesia, both highlands and lowlands, contain 3-n-butylphthalide, D-limonene, Linalool, Luteolin, Apigenin and Apiin compounds. Differences in geographical location of growth resulted in differences in the content and concentration of Apium graveolens L. Aim and Objectives: This study was conducted to determine the differences of Apium graveolens L. based on its distribution in Indonesia. Indonesia does not yet have a standard for Apium graveolens L. Material and Methods: this research uses Apium graveolens provided by the Center for Research and Development of Traditional Medicinal and Medicinal Plants (B2P2TOOT) Tawangmangu. 10 samples from different regions in Indonesia will be read using the ATR-FTIR instrument in the finger print area as well as PCA (principal principal) multivariate analysis. component analysis) and HCA (Hierarchical Clustering Analysis). Results: The PCA results show the highest number of variants on PC 1 91,98% and PC 2 variants at 4,71%. In the quadrant 4 score plot, it was found that there was a closeness between the standard sample and the S2 sample (Materia Medika Batu). Conclusion: The result of this study is that there is a mutually supportive relationship from spectrum readings, sample location (temperature, soil moisture content, height (m a.s.l) with multivariate analysis (PCA, HCA results) when compared to the geographic location of each sample.