Antibacterial Effect of Nigella sativa L. Seed from Indonesia

Nigella sativa L. was allegedly to has antibacterial effect. The aim of this research is to investigate antibacterial effect of ethanolic extracts of 96% Nigella sativa L. from Indonesia toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis. The Antibacterial activity of of Nigella sativa L toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis using the well method. The 96% ethanolic extract of 12,5; 25;50 and 100% (mg/ml)inhibit growth of Staphylococcus aureus (with inhibition zone 11.06; 29.58; 28.22; 30.84 mm respectively) and Streptococcus epidermidis (20.90; 31.90; 29.93; 33.07 mm respectively). Ethyl acetate fraction of 96% ethanolic extract of Nigella sativa concentration of 6,25; 12,5; 25;50 and 100% (mg/ml) inhibit growth of Staphylococcus aureus (12.91; 15.06; 19.19; 37.48; 46.18 mm respectively and Streptococcus epidermidis (19.07; 19.21; 20.22; 21.62; 40.00 mm respectively). Ethanolic 96% extract and ethyl acetate fraction of ethanolic extract of Nigella sativa have antibacterial effect toward Staphylococcus aureus and Streptococcus epidermidis invitro.