ArticleViewAbstractPharmacognosy Journal,2023,15,1,128-133.DOI:10.5530/pj.2023.15.17Published:March 2023Type:Research ArticleThe Effect of Drying Methods on Chemical Profiling of Zingiberaceae Herbs ProductionDinar Sari C. Wahyuni, Siti A. E. Astuti, Soerya Dewi Marliyana, Yudi Rinanto, Tiara Dewi S. Pratama, Ahmad Fauzi, and Ari Satia Nugraha Dinar Sari C. Wahyuni1,*, Siti A. E. Astuti1, Soerya Dewi Marliyana2, Yudi Rinanto3, Tiara Dewi S. Pratama1, Ahmad Fauzi4, Ari Satia Nugraha5 1Pharmacy Department, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57 126, Central Java, INDONESIA. 2ChemistryDepartment, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57 126, Central Java, INDONESIA. 3Biology Education Program, Faculty of Teacher Training and Education, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57 126, Central Java, INDONESIA. 4Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani, Pabelan, Kartasura, Sukoharjo 57169, Central Java, INDONESIA. 5Drug Utilisation and Discovery Research Group, Faculty of Pharmacy, University of Jember, Jl. Kalimantan I/2, Jember, 68121, East Java, INDONESIA. Abstract:Background: Drying method is one of the important steps in post-harvesting in herbal production in order to prolong the shelf-life of herbal medicine and to ensure the good quality. Objective: This research aimed to profile and quantify the metabolites changing using metabolomic based on 1H-NMR Spectroscopy on Curcuma and Zingiber genus due to the drying process. Material and Methods: Five species of the Curcuma and Zingiber genus were Curcuma longa L., Curcuma aeruginosa Roxb., Curcuma xanthorrhiza Roxb., Zingiber officinale Roscoe. and Zingiber amaricans BI. The drying method applied were freeze drying and oven drying method at -1080C and 500C, respectively. Samples were extracted using buffer phosphate and deuterated methanol (3:7). The analysis was performed using 1H-NMR spectroscopy 400 MHz with TSP as an internal standard. The 1H-NMR spectra were analysed with multivariate (Principal Component Analysis). Results: The relative concentrations of sucrose and fructose at 5 samples in freeze drying method showed significant higher content than those in oven drying. On the other hands, glucose was mostly significant higher concentration in oven drying at 5 samples. Fructose and sucrose were significantly higher in freeze drying method than those in oven drying in Curcuma and Zingiber genera. Relative glucose content was higher in oven drying at Curcuma genus Conclusion: Therefore, oven drying method which applied heating at 500C is an appropriate method for herbal medicine production, especially in Curcuma and Zingiber genus, for maintaining the major secondary metabolites. Keywords:Curcuma Genus, Freeze drying, Multivariate, Oven drying, Zingiber Genus.View:PDF (500.97 KB) PDF Images Column plot PCA of PC5 1H-NMR spectra in the extract of Curcuma and Zingiber involving oven and freeze-drying methods. Figure ‹ Anticancer and Neuroprotective Effects of the Triterpene Glycosides From Sea Cucumber Holothuria imitans up Sun Protection Factor Activity of Jamblang Leaves Serum Extract (Syzygium cumini) ›