Impact of Stigmasterol from Beluntas Leaves (Pluchea indica) on SGOT and SGPT Levels in Male Rats (Rattus norvegicus)

Stigmasterol compounds have the potential as natural male antifertility drugs; based on the results of multiyear research and bioinformatics, further research is needed on the safety of stigmasterol preclinically. The study aimed to examine selected stigmasterol from blunt leaves that are safe for blood SGOT and SGPT levels the type of experimental research, the Post-test control design group. The study was conducted at the Biomedical Laboratory of the University of Muhammadiyah Malang, the Hufaka Bandung Laboratory, and the FKH Laboratory of Brawijaya University. The study was conducted from June to August 2024. The research sample of stigmasterol from blunt leaves came from the UPT materia medica of Batu City. The sampling technique was purposive random sampling. There were three dose treatments (0.125 mg/kgbb, 0.25 mg/kgbb, 0.5 mg/kgbb and control) with seven replications. Data analysis used one-way ANOVA and Duncan's further test. The results showed that giving various doses of stigmasterol could reduce SGPT in the blood but not SGOT. Duncan's test results of SGOT levels showed no significant difference between treatment categories; SGPT testing showed that the 0.125 mg/kgbb and 0.25 mg/kgbb treatments were not significantly different from the 0.5 mg/kg bb treatment but were substantially different from the control treatment. At the same time, the 0.5 mg/kgbb treatment was not significantly different from the other treatments. The conclusion and findings of the stigmasterol compound are in the safe category for use because it does not cause an increase in blood SGOT and SGPT and potentially tends to decrease.