ArticleViewAbstractPharmacognosy Journal,2025,17,2,203-210.DOI:10.5530/pj.2025.17.25Published:April 2025Type:Original ArticleAcute and Subchronic Toxicity Study of Sampilnorov WurileLi ChangSheng, Chimgee Tserendash, Maral-Erdene Gerelt-Od, Batdavaa Batbayar, Uyangamaa Altankhuyag, Mend-Amar Batbaatar, Narankhajid Myadagsuren, Davaadagva Damdinjav, Enkhmaa Gonchig, and Yeruult Chultemsuren Li ChangSheng1, Chimgee Tserendash2, Maral-Erdene Gerelt-Od2, Batdavaa Batbayar2, Uyangamaa Altankhuyag2, Mend-Amar Batbaatar2, Narankhajid Myadagsuren3, Davaadagva Damdinjav4, Enkhmaa Gonchig2,*, Yeruult Chultemsuren2,* 1Department of Mongolian Medicine Surgery, College of Mongolian Medicine, Inner Mongolia Medical University, China; No 5 Xinhua Street, Hohhot, Inner Mongolia Autonomous region, MONGOLIA. 2Department of Pharmacology, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MONGOLIA. 3Department of Biology, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MONGOLIA. 4School of Pharmacy, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MONGOLIA. Abstract:Introduction: Sampilnorov Wurile (SW) is a traditional medicinal preparation containing 29 plant-, mineral-, and animal-derived components. It is used for cerebrovascular ischemia, retinal disorders, cardiovascular diseases, and neurological conditions. This study aimed to evaluate the acute and subchronic toxicities of SW. Methods: Acute toxicity was assessed in C57BL/6 mice based on OECD-423 guidelines, with oral doses ranging from 500 to 6,000 mg/kg body weight. Subchronic toxicity was evaluated in Wistar rats following OECD-407 guidelines, with daily oral doses of 300 and 600 mg/kg of SW daily for 4 weeks. Clinical signs, mortality, body weight, and physical condition were monitored. Hematological, biochemical, and histopathological analyses were conducted on day 29. Results: No mortality or significant clinical signs of toxicity were observed at doses up to 6,000 mg/kg, suggesting an oral median lethal dose exceeding this level. Subchronic toxicity assessment revealed no clinical signs of toxicity or mortality. SW at 300 and 600 mg/kg had no significant effects on serum biochemical parameters or vital organ histology compared to controls. Conclusion: SW exhibited no acute toxicity at doses up to 6,000 mg/kg and showed no adverse effects in a 28-d subchronic toxicity study. These findings support its safety at tested doses. Keywords:Acute and subchronic toxicity; biochemistry; blood test; histopathological analysis; Sampilnorov WurileView:PDF (718.82 KB) PDF Images Body weights measurements in mice administered Sampilnorov wurile. ‹ Housing In An Enriched Environment Enhances The Neuroprotective Effect Of Celastrus Paniculatus And Tribulus Terrestris In An Animal Model Of Chronic Stress up Chemical Composition and Nutritional Value of Edible Moroccan Truffles ›