HPLC Quantification of Hydroxycinnamic and Organic Acids of Canadian Goldenrod (Solidago canadensis L.)

Background: Canadian goldenrod (Solidago canadensis L.) is a medicinal plant widely used in traditional medicine across the world for several hundred years. According to literature data, S. canadensis contains various groups of biologically active substances, including tannins, flavonoids, etc. The aim of the study was to identify and quantify hydroxycinnamic and organic acids in aerial parts of Canadian goldenrod, as these groups of substances demonstrate a broad spectrum of therapeutic activities. Materials and Methods: Ethanolic extracts of S. canadensis, gathered in Central Russia, were analyzed using highperformance liquid chromatography (HPLC). Hydroxycinnamic acids (HCA) determination was carried out by HPLC method with UV detection at 330 nm using HCA reference standards (RS). Organic acids (OA) determination was performed in the same manner, utilizing UV detection at 210 nm and corresponding OA RS. Results: It was established that S. canadensis HCA composition is represented by cichoric, caffeic, chlorogenic, quinic and ferulic acids. The total HCA content in was 1.16 g ± 10.7 mg / 100 g. Main OA, found in S. canadensis, are ascorbic, citric, tartaric, succinic, gallic, malic, oxalic and fumaric acids, with the total OA content of 426.5 mg ± 6.4 mg / 100 g. Conclusion: The described HPLC method was successfully used for analysis of S. canadensis aerial parts ethanolic extracts. The method can be utilized for HCA and OA identification and quantification in both herbal raw material and herbal medicinal products containing Canadian goldenrod.