ArticleViewAbstractPharmacognosy Journal,2021,13,1,172-178.DOI:10.5530/pj.2021.13.24Published:January 2021Type:Research ArticleAnalysis of Several Inflammatory Markers Expression in Obese Rats given Plectranthus amboinicus (Lour.) Spreng Ethanol ExtractKarnirius Harefa, Delmi Sulastri, Ellyza Nasrul, and Syafruddin Ilyas Karnirius Harefa1, Delmi Sulastri1, Ellyza Nasrul1, Syafruddin Ilyas2,* 1Study Programme of Biomedic, Faculty of Medicine, Universitas Andalas, Padang, INDONESIA. 2Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Sumatera Utara, Medan, INDONESIA. Abstract:Introduction: Oxidative stress is one of the inflammatory events caused by obesity. This condition is characterized by an increase in various inflammatory markers, such as intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and a cluster of differentiation 40 (CD40). This study aimed to analyze the effect of Plectranthus amboinicus (Lour.) Spreng ethanol extract on ICAM-1, VCAM-1, and CD40 in obese rats. Methods: The study used a pure experimental method with a completely randomized design. There were 6 groups, namely, C− (negative control), C+ (positive control), CMC (soluble control), EE300 (P. amboinicus ethanol extract, 300 mg/kg body weight [BW]), EE600 (P. amboinicus ethanol extract, 600 mg/kg BW) and EE900 groups (P. amboinicus ethanol extract, 900 mg/kg BW). Results: The results showed low levels of ICAM-1 and VCAM-1 in the blood plasma, especially in the EE900 group, but the difference was not substantial. The same trend also occurred in the expression of CD40 in the tunica intima layer of the rat aorta. Conclusions: Thus, the administration of 900 mg/kg BW P. amboinicus ethanol extract for 45 days has the potential to treat obesity in rats through the suppression of oxidative stress and inflammatory markers (ICAM-1, VCAM-1 and CD40). Keywords:Enzyme-Linked Immunosorbent Assay, immunohistochemistry, Obese, Plant extract, RatsView:PDF (1.75 MB) PDF Images Graphical Abstract ‹ GC-MS Analysis of Bioactive Compounds and Safety Assessment of the Ethanol Extract of the Barks of Holarrhena pubescens Wall. ex.G.Don (Family Apocynaceae): Sub-Acute Toxicity Studies in Swiss Albino Mice up Liver Organ Impairment Due to the Consumption of Kratom Leaves (Mitragyna speciosa Korth.) ›