ArticleViewAbstractPharmacognosy Journal,2023,15,6,1116-1120.DOI:10.5530/pj.2023.15.203Published:December 2023Type:Research ArticleExtract of Torbangun (Coleus amboinicus Lour) Leaves Inhibits the Expression of PEPCK and G6Pase in Streptozotocin-Induced Diabetic RatsMeilla Dwi Andrestian, Rizal Damanik, Faisal Anwar, Nancy Dewi Yuliana, and Anwar Mallongi Meilla Dwi Andrestian1,*, Rizal Damanik2,3, Faisal Anwar2 , Nancy Dewi Yuliana4 , Anwar Mallongi5 1Department of Nutrition, Polytechnic of Health Ministry of Health Banjarmasin, Banjarbaru, South Kalimantan, INDONESIA. 2Department of Community Nutriton, Faculty of Human Ecology, IPB University, Bogor, INDONESIA. 3Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center Bogor, IPB University, Bogor, INDONESIA. 4Department of Food Science, Faculty of Agricultural Agricultural Engineering and Technology, IPB University, Bogor, INDONESIA. Abstract:Objective: This study aimed to analyze the anti-hyperglycemic activity of Torbangun leaf extract (TLE) in hyperglycemic rats by observing its effect on blood glucose levels and PEPCK and G6Pase gene expression. Method: This post-test-controlled group design study used a completely randomized design (CRD). Seven rats were separated as a normal group (N) and other rats were injected with streptozotocin (STZ). Hyperglycemia was characterized by fasting blood glucose (FBG) >126 mg/dl. Hyperglycemic rats were divided into three groups: H-IM (control drug metformin hydrochloride dose 62.5 mg/kg BW), H-IT (TLE dose 620mg/kg BB), and NG (negative control, hyperglycemic rats). Rats were given intervention for 14 days. Result: The results showed a significant difference between initial and final FBG of the HI-IM group (p = 0.002, α = 0.01) and the H-IT group (p = 0.005, α = 0.01) and effect on the key gene expression of gluconeogenesis in the liver. The H-IT group showed a decrease in PEPCK gene expression 0.80 times and decreased G6Pase gene expression by 0.65 times compared to NG group. Conclusion: The findings suggested the administration of TLE in STZ-induced hyperglycemic rats was able to reduce blood sugar levels and was involved in inhibiting the expression of PEPCK and G6Pase expression genes in the liver. Keywords:G6Pase, hyperglycemia, PEPCK, Rats, TorbangunView:PharmacognJ-15-6-1116.pdf (230.08 KB) PDF Images PEPCK and G6Pase gene expression at transcription level ‹ Role of 1,25(OH)2D On Cytochromes CYP27A1 and CYP27B1 in Periodontitis: A Clinical Study up The Mediating Role of Perceived Value on the Relationship between Quality Assurance and Student Satisfaction in Nursing Education ›