ArticleViewAbstractPharmacognosy Journal,2024,16,1,126-130.DOI:10.5530/pj.2024.16.17Published:February 2024Type:Research ArticlePunicalagin Opposes Gentamicin Nephrotoxicity in Rats: Role of Nrf2 and NF-κB PathwaysAmr A. Fouad, Moataz Mohamedalhasan Ali, and Mostafa Abdel-Hamid Amr A. Fouad1,*, Moataz Mohamedalhasan Ali2, Mostafa Abdel-Hamid3 1Department of Pharmacology, Faculty of Medicine, Al-Baha University, Al-Baha, SAUDI ARABIA. 2Department of Pathology, Faculty of Medicine, Al-Baha University, Al-Baha, Saudi Arabia & Department of Pathology, Faculty of Medicine, University of Elimam Elmahdi, SUDAN. 3Department of Anatomy and Embryology, Faculty of Medicine, Al-Baha University, Al- Baha, SAUDI ARABIA. Abstract:Background: Oxidative stress, inflammation, and apoptosis are implicated in gentamicin (GEN)-induced nephrotoxicity. Punicalagin (PNG) possesses antioxidant, anti-inflammatory, and antiapoptotic effects. Objective: The aim of the present research was to investigate the possible defensive effect of PNG against nephrotoxicity caused by GEN in male Sprague-Dawley rats. Materials and Methods: GEN (80 mg/kg/day, i.p.) was administered for 8 days. Treatment with PNG (25 mg/kg/day, p.o.) for 10 days, began 2 days before GEN insult. Results: PNG significantly decreased serum creatinine, and malondialdehyde, tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, nuclear factor-κB p65 (NF- κB p65), and cleaved caspase-3 activity in the kidneys of GEN-challenged rats. PNG also significantly increased renal catalase, reduced glutathione, and nuclear factor erythroid 2-related factor 2 (Nrf2) in rats received GEN. Additionally, PNG markedly attenuated the histopathological kidney tissue injury caused by GEN. Conclusion: PNG guarded against GEN-induced kidney damage in rats through its antioxidant, anti-inflammatory, and antiapoptotic effects, and by modulating the balance between Nrf2 and NF-κB pathways. Keywords:Gentamicin, Kidney, Punicalagin, RatsView:PDF (1.1 MB) PDF Images Effect of punicalagin (PNG) treatment on serum creatinine of gentamicin (GEN)-challenged rats. ‹ Pharmacognostic Profile and Antidiabetic Activity of Eleutherine bulbosa Mills. Bulbs from East Kalimantan, Indonesia up Screening and Identification of Metabolites from Sambiloto (Andrographis paniculata) Ethanol Extract for Pro-Inflammatory Cytokines Inhibitory through In Silico and In Vitro Approaches ›