Introduction: System injury, manifested as metabolic dysfunction is a common side effect of chemotherapy. Chemical stress on hepatic and kidney cells can be evaluated by biochemical analysis, including serum aminotransferases, glutathione levels and extent of lipid peroxidation. Combined treatment of chemotherapy along with the natural products such as phytochemicals may be a newer approach to reduce the side effects and betterment of chemotherapy. Objective: The present study was conducted to assay kidney and liver associated parameters on animals exposed to etoposide, and the protective effect of different doses of Spondias pinnata bark extract on normal cells. In this study, total 24 male Wistar rats (either sex) aged between 60 to 90 days were considered. Each groups consisted of six rats. Levels of Alanine and Aspartate aminotransferases were estimated using semi-auto analyser and GSH, GST, TAO and LPx by spectrophotometry. Results and Conclusion: Data analyzed using Graph Pad 5.0. Variation in the analyzed parameters were found between control, chemotherapy and Spondias pinnata groups. Results of the study show that significant increase in TBARS levels indicative of etoposide induced oxidative stress could be prevented by the treatment with S. pinnata. GST levels also support the preventive action of S. pinnata against etoposide induced stress. However, TAO and transaminases remain unaltered in the study groups.