Production of Secondary Metabolites and its Antibacterial and Antioxidant Activity During the Growth Period of Endophytic Fungi Isolated from Gall Rust Sengon Plants

Introduction: In our previous study, secondary metabolites of endophytic fungi isolated from gall rust sengon showed their bioactive activity against antibacterial and antioxidant, but only gave little effect to antifungal activity. Endophytic fungal culture extracted from gall rust of sengon (Falcataria moluccana Miq. Barneby and J. W. Grimes) has the ability on inhibiting Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. To increase the production of bioactive compounds, this research was focused on isolates which produced the highest activity compounds, and sought the optimal fermentation conditions in the production of bioactive compounds. Methods: The fermentation process was carried out on PDB liquid media for 21 days, under shaker conditions, at room temperature, and sampling measurements were hold every 3 days. Bioassay were carried out against B. subtilis, P. aeruginosa, and E. coli. Results: The early stages fungal growth was adaptation stage, continued by 1-6^th days of exponential growth period. Day 6-12^th was stationary growth and day 15-21^th were cell death periode. The highest secondary metabolite production was achieved at stationary periodes, that was occured at days 9-15^th and high antibacterial activity was produced on days 9-12^th. Conclusion: The best production secondary metabolite that has high activity was at day 9-12^th.