Introduction: Garcinia latissima Miq known as Dolo magota (Maluku), is a medicinal plant belonging to the family Clusiaceae. The purpose of the research was to explore the phytoconstituents present, pharmacognostic details, and their antimicrobial efficacy. Methods: The preliminary phytochemical components were qualitatively examined using the standard method systems. The antimicrobial screening was carried out using the good diffusion method and the minimum inhibitory concentration (MIC) using dilution method. Results: The phytochemical screening of different extract of G. latissima Miq leaves revealed the presence of tannins, saponins, and alkaloids and the results were tabulated. The ethyl acetate and methanolic extracts from its leaves showed antimicrobial activity especially for Bacillus subtilis, a positive bacteria; the hexane extract did not show any activity against the selected microba. Conclusion: The results of the phytochemical and bio-efficacy study revealed most valuable information and also support the continued sustainable use of this leaves in the traditional system of medicine.