Objectives: Since the beginning of human civilization various herbal medicines are employed for healing human and animal. Pogostemon cablin (Blanco) Benth., locally known as Patchouli (Assamese) is a very important medicinal plants belongs to mint family i.e. Lamiaceae. The main aim of this study was to collect ethnobotanical information’s and to study its in vitro regeneration results. All possible ethnobotanical literatures have been cited here. Methods: In vitro propagation was achieved from leaf and nodal explants of Pogostemon cablin on MS medium. Results: Callus development and in vitro axillary shoot formation was successfully made in MS basal medium containing BA (4.0 mg/L), NAA (2.0 mg/L)+ IAA (1.0 mg/L) and BA (3 mg/L)+IAA (1 mg/L). MS basal medium containing IBA (0.1/L) and Kn (2.5 mg/L) was best for induction of multiple shoots within 4 weeks of culture. Combination of NAA (0.1 mg/L). Kn (0.1 mg/L) and CH (100 mg/L) was best for callus induction which later on formed multiple shoots and caused elongation of roots. Micro shoots of varied length were produced on MS medium. Rooted plantlets were successfully acclimatized in green house for 1 month and then were transferred to the field. Conclusion: It can be concluded that pogostemon cablin has immense ethno botanical importance. For its rapid multiplication, in vitro technique was found very successful. In MS medium supplemented with Kn 2.5 mg/L and IBA 0.1mg/L found maximum multiplication rate. In this proportion rates of shoot generation, leaf, rooting, callus formation was maximum.