ArticleViewAbstractPharmacognosy Journal,2019,11,6s,1463-1470.DOI:10.5530/pj.2019.11.226Published:November 2019Type:Original ArticleImpact of Solvent on the Characteristics of Standardized Binahong Leaf (Anredera cordifolia (Ten.) Steenis)Dwitiyanti, Yahdiana Harahap, Berna Elya, and Anton Bahtiar Dwitiyanti1, Yahdiana Harahap2, Berna Elya3, Anton Bahtiar4,* 1Graduated Program of Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java 16424, INDONESIA. 2Department of Bioanalysis and Bioequivalence, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java 16424, INDONESIA. 3Department of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java 16424, INDONESIA. 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java 16424, INDONESIA. Abstract:Background: Binahong is a plant that has the potential to be used as a traditional herbal medicine in Indonesia, and has several kinds of classes of compounds, one of them is a flavonoids glycosides (vitexin). Previous research reported that binahong leaves have pharmacological activities as antihyperglycemic, antihyperlipidemic, antibacterial, and others. A traditional plant that has proven efficacious needs to be standardized to ensure the quality and its safety. Objective: This study aimed to characteristics of binahong leaves simplicia obtained from Bogor, West Java. Materials and Methods: The crude extract was obtained by the maceration method using 40%, 70%, and 96% ethanol solvent. The selected extract then standardized, which includes macroscopic and microscopic observations and sets the standard parameter values binahong leaf extract. Parameters LCMS to identify active compounds semiquantitatively. Results: The yield of binahong ethanol extract from 40%, 70%, 96% showed a value of 10.9%, 11.4%, and 12.32%, respectively. From these results, 96% ethanol extract has proceeded for standardization. Macroscopic observation results showed that binahong leaves simplicia has a fine and notched form with 5-10 cm long and 3-7 cm diameter. The microscopic binahong leaves contain palisade tissue, parenchymal tissue, chlorophyll grains, rosette Caoxalate crystals, and spiral type. Phytochemical screening of binahong leaves showed the presence of alkaloids, flavonoids, saponins, tannins, steroids, and phenolic compounds.The standardization of binahong leaves ethanol extract down showed a levels of ethanol-soluble extract> 14.8%, water-soluble extract content > 13.5%, drying < 10%, water content < 8.9%, total ash content < 7.2%. LCMS profiles showed that ethanolic extract 40%, 70%, and 96% all contained vitexin at retention time 5.02 minutes, and m/z values 433.1111. Conclusion: 96% ethanolic extract of binahong leaves contains vitexin with pharmacognostic parameters carried out following the standards listed in the Indonesian herb pharmacopeia. Keywords:Anredera cordifolia, Extract, LCMS, Simplicia, standardizationView:PDF (1.75 MB) PDF Images (a) 1. Epidermis, (b) 1. Stomata, 2. Chlorophyll, 3. Cell closing, (c) 1. Ca-Oxalate crystal in rosette, (d) 1. Thickening of the carrier beam spiral shape ‹ Influence of Extraction on the Yield, Phytochemical and LCMS Profile from Standardized Kemuning Leaf (Murraya paniculata (L.) Jack) up Study of Molecular Docking of Vitexin in Binahong (Anredera cordifolia (Ten.) Steenis) Leaves Extract on Glibenclamide-CYP3A4 Interaction ›