ArticleViewAbstractPharmacognosy Journal,2025,17,1,58-62.DOI:10.5530/pj.2025.17.7Published:January 2025Type:Research ArticleEffect of Different Extraction Solvents on the Total Phenolic Content and Antioxidant Activity of Brassica oleracea var. italicaVivian Nathaly Echegaray Florian, Claudia Giuliana Montoya Sisniegas, and Juan Ernesto Valdiviezo-Campos 1Estudiante de la Escuela de Nutrición, Facultad de Ciencias de la Salud, Universidad César Vallejo, Av. Larco 1770, Trujillo, PERÚ. 2Grupo de investigación en Productos Naturales. Escuela de Nutrición, Universidad César Vallejo, Av. Larco 1770, Trujillo, PERÚ. Abstract:This study offers an alternative solution for the prevention and/or treatment of diseases caused by free radicals. The objective was to evaluate the effect of different solvents on the total phenolic content and antioxidant activity of Brassica oleracea var. italica (broccoli). Methods: The sample, consisting of stems and florets of Brassica oleracea var. italica (broccoli), were collected from the Chocas community in Carabayllo, Lima, Peru. Three extracts were prepared using different solvents, including a mixture of ethanol and water. The total phenolic content was determined using the Folin-Ciocalteu method, and antioxidant capacity was evaluated using two specific assays (DPPH and ABTS). Additionally, the correlation between total phenolic content and antioxidant activity was analyzed. Results: The hydroethanolic extract demonstrated the highest phenolic content, with 686.02 mg GAE/100 g dry matter). It also exhibited strong antioxidant activity, measuring 1035.81 mg TE/100 g DM in the DPPH assay and 6506.94 mg TE/100 g DM in the ABTS assay. Conclusion: The highest total phenolic content and antioxidant activity of Brassica oleracea var. italica were found in the hydroethanolic extract, which showed a significant high correlation. Frequent consumption of broccoli in the diet is recommended due to its high values. Keywords:Antioxidant, Broccoli, Extract, Polyphenol, VegetableView:PDF (404.15 KB) PDF Images Correlation analysis between total phenolics and antioxidant activity of B. oleracea var. itálica. ‹ The In-Vitro Antiviral Activity of Carissa Edulis, Tulbaghia Acutiloba, and Tetradenia Riparia on Severe Acute Respiratory Syndrome Coronavirus-2 up Microencapsulation of Paracetamol with Polycaprolacone Coating ›