Screening of Phytochemical Analysis and In vitro Bioactive of Polyherbal Formulation

Introduction: Plants have the capability to synthesize various forms of phytochemical compounds as secondary metabolites. Method: In the present investigation phytochemicals such as alkaloids, tannins, glycosides, carbohydrates, reducing sugar, proteins, saponins, flavanoids, phenols, terpenoids and phytosterols were detected in polyherbal formulation A. marmelos, G. glabra and R. centrifolia. Results: The Total flavonoid, steroids, alkaloids and phenolic content was observed from fruit, root and pettles1.40mg QE/g DE, 12.14mg BE/g DE, 14.40mg AE/g DE and 99.33mg GAE/g DE. FTIR spectrum of the polyherbal sample revealed 5 major peaks at 2919.40 cm^-1, 2357.62 cm^-1, 1150.56 cm^-1, 1076.22 cm^-1 and 1015.64 cm^-1. The antibacterial activity was maximum zone of inhibition (19 mm) was recorded in S. aureus strain and minimum zone of inhibition (5mm) was observed in S.mutans strain. The antioxidant study maximum and minimum scavenging DPPH, NOR, H2O2 and SOD activities (%) of 62.28, 53.68, 39.67 & 43.98 at 5 mg/ml and 39.88, 36.49, 5.9 and 34.94 at 1mg/ml was recorded. The polyherbal sample exhibited significant albumin denaturation, proteinase inhibitory, membrane stabilization and lipid peroxidation inhibitory activities as the maximum inhibition of 46.53%, 36.7%, 51.9% and 64.71% was observed at 500 μg/ml. Conclusion: Hence the present studies indicate good antibacterial, antioxidant and anti-inflammatory activities from the medicinal plants, A. marmelos, G. glabra and R. centrefolia proves the possibility of its utilization as an additional potent source medicinal uses.