In vitro Acetyl cholinesterase inhibitory assay of Acacia catechu Willd ethanolic seed extract

Aim & objective: The aim of this study was to evaluate acetyl cholinesterase inhibitory activity of Acacia catechu ethanolic seed extract to introduce a new source for management of Alzheimer’s disease. Background: Alzheimer’s disease is a complex, multifactorial, progressive, neurodegenerative disease primarily affecting the elderly population and is estimated to account for 50–60% of dementia cases in persons over 65 years of age. It is likely that the inhibition of acetyl cholinesterase by Acacia catechu ethanolic seed extract that is rich in flavonoids and antioxidants may aid in the protection of neurodegenerative disorders and ultimately Alzheimer's disease. Methods: Anti cholinesterase activity is determined by adopting In vitro standard protocol. Results: According to the obtained results, the inhibitory activity (IC₅₀ values, μg /ml) of extracts was 204.38 ± 2.54 µ g/ml. Conclusion: The results indicated and confirmed the traditional use of Acacia catechu ethanolic seed extract for management of central nervous system disorders. It showed the moderate activity in inhibition of acetyl cholinesterase at various concentrations. However, further investigations on identification of active components in the extracts are needed.

Key words: Acacia catechu seed, Acetyl cholinesterase inhibitor, Alzheimer’s disease, spectrophotometric analysis.