Chitosan-Tripolyphosphate Nanoparticles of Mango Ginger (Curcuma mangga) Extract: Phytochemical Screening, Formulation, Characterization, and Antioxidant Activity

Introduction: Mango ginger (Curcuma mangga) is one of Indonesia's medicinal plants widely used in most communities as a lust booster and for detoxifying purposes. Therefore, the purpose of this study is to synthesize chitosan-tripolyphosphate nanoparticles from mango ginger extract, determine their chemical contents, the nano chitosan characteristics, and its antioxidant activity. Methods: In this study, we macerated mango ginger using 70% ethanol solvent, then performed phytochemical test and formulation of chitosan nanoparticles of mango ginger extract. The group of secondary metabolites that showed positive results with the reagent test was further identified through TLC. Results: The results showed that the extract contained flavonoids and triterpenoids. Also, characterization of chitosan nanoparticles from the extract was conducted with FTIR test, PSA, XRD, and SEM. Based on the results, the nano chitosan particle size was 993 nm and examination with FTIR showed the presence of N-H and P=O groups, indicating ammonium ion interaction from chitosan with the polyanion from TPP and Mango ginger. Additionally, the XRD results showed that the crystals formed were in an amorphous form, which was supported by particle morphology images from SEM. Furthermore, the nanoparticles showed very strong antioxidant activity based on the reaction with DPPH. Conclusion: Based on these results, the phytochemical identification of mango ginger extract showed positive results in flavonoid and triterpenoid compounds. In addition, based on the characterization of the nanoparticles, the mango ginger extract showed positive results, illustrating that the nano chitosan synthesis was successful. Furthermore, the nano chitosan has a very strong antioxidant activity with an IC50 value of 18.08 μg/mL.