Effects of High Intensity Swimming on Heat Shock Protein 70, Superoxide Dismutase and Malondialdehyde of Rattus norvegicus Male Rats

Introduction: This study aims to analyze relationship between physical activity with high intensity swimming by proving the difference in the mean levels of HSP-70, SOD levels and MDA levels in male white rats (Rattus norvegicus) who perform vigorous-intensity physical activity compared to male white rat (Rattus norvegicus) who do not engage in strenuous physical activity. Method: This research is a laboratory experimental research. This research is a True Experimental Research type that uses a Post Test Only Control Group Design research design. The experimental unit consisted of 28 white rats (Rattus norvegicus). The study was started by dividing the rats into 2 groups. Group 0 who did not receive heavy physical activity treatment and group 1 who received heavy intensity physical activity treatment 3 times a week for 4 weeks. Results: The results of the Shapiro-Wilk test showed that the HSP-70 data were normally distributed (p<0.05). SOD and MDA data for all groups were normally distributed (p>0.05). The results of the Kruskal Wallis test showed that there was a significant difference in HSP-70 between groups (p > 0.05), the results of the Mann Whitney test showed that the HSP-70 of the K0 group was significantly different from the K1 group. The results of the analysis of variance with Brown-Forsythe showed that there was a significant difference in SOD between groups (p < 0.05). The results of the Games Howell test showed that the SOD of group K0 was significantly different from that of group K1. The results of the analysis of variance showed that there was a significant difference in MDA between groups (p < 0.05). The results of the LSD test showed that the MDA of the K0 group was significantly different from the K1 group. Conclusion: There is a difference in the mean levels of HSP-70 in white male rat (Rattus norvegicus) whoperform strenuous physical activity, lower than male white rat (Rattus norvegicus) who do not engage in strenuous physical activity. There is a difference in the mean SOD levels in male white rat (Rattus norvegicus) which perform strenuous physical activity, lower than male white rat (Rattus norvegicus) who do not do strenuous physical activity. There is a difference in the mean MDA levels in male white rat (Rattus norvegicus) after doing strenuous physical activity, higher than male white rat (Rattus norvegicus) who do not engage in strenuous physical activity.