Evaluation of Antioxidant Activity and Flavonoid and Phytocompounds Content in Pongamia Pinnata Seed

Introduction: Pongamia pinnata is a woody shrub and tree found in entire India mainly in dry areas. The seeds of P. pinnata are collected and traditionally oil is extracted and used to kill helminthes. The plant is a remedy for skin diseases, wound, rheumatism & ulcers and also as biofuel, green manure and fodder. This study is an attempt to know antioxidant activity of particular fraction which is having antifungal activity and flavonoid and fatty acids content which is responsible for the same. Materials and methods: In this investigation, an effort was made to know the antioxidant activity of PPEAF by DPPH assay, FRAP assay and H₂O₂ assay methods and Optimization of mobile phase by TLC method and supporting major flavonoid content quantification by HPTLC method Results: The result shown the ethyl acetate fraction has significant antioxidant activity of IC₅₀ value of 212.09 (DPPH assay), 235.87 (FRAP assay) and 268.62 (H₂O₂ assay). The TLC method optimum mobile phase was found to be Toluene: ethyl acetate (7:3). The standard flavonoids used are Karanjin, Gallic acid and Quercetin. Conclusion: The HPTLC method results shown PPEAF was found to contain 1.465% Karanjin. GCMS result shown the PPEAF contain 13 phytocompounds in which Octadec-9-enoic acid at retention time 22.356 was found to be abundant.