ArticleViewAbstractPharmacognosy Journal,2023,15,4,599-605.DOI:10.5530/pj.2023.15.126Published:August 2023Type:Research ArticleAntioxidant, Total Phenolic, and Total Flavonoid of 70% Ethanol Extract of Avocado Seeds (Persea americana Mill.)Sri Wahyu Ningsih Munthe, Riskianto Riskianto, Denny Juvi, and Jessica Novia Sri Wahyu Ningsih Munthe, Riskianto Riskianto, Denny Juvi, Jessica Novia* Pharmacy, Faculty of Health Sciences, Universitas Pelita Harapan, Tangerang, INDONESIA. Abstract:Avocado seeds (Persea americana Mill.) are high in phytochemicals and are utilized in herbal medicine. The objective of this study is to analyze the antioxidant activities, total phenolics and flavonoids of the P. americana seed extract. P. americana seed extraction was obtained through maceration and reflux using a 70% ethanol solvent. The results obtained were compared in terms of yield productivity, with yields of 43.07 (%) and 39.58 (%) respectively. Phytochemical compounds extracted from P. americana seeds were tested using the phytochemical screening method, the antioxidant activity assay, the total phenolic analysis, and the total flavonoid analysis. The phytochemical screening showed that P. americana seeds contain flavonoids, saponins, phenols, tannins, alkaloids, and quinones. The antioxidant activity of the 70% ethanol extract of P. americana seeds obtained by maceration and reflux method was 77.298 g/mL and 98.626 g/mL, respectively, meanwhile the IC50 values of vitamin C were 12.883 g/mL. The 70% ethanol extract of P. americana seeds obtained by maceration and reflux method had total phenolic content of 276.96 mgGAE/g and 294.96 mgGAE/g, and total flavonoid content of 1.73 mgQE/g and 12.70 mgQE/g respectively. This simply implies that the 70% ethanolic extracts from P. americana seeds obtained through maceration and reflux have strong antioxidant activity. Keywords:Antioxidants, Avocado seeds, DPPH, Persea americana Mill., Total flavonoids, Total phenolics.View:PDF (480.91 KB) PDF Images Graphical Abstract ‹ Anti-inflammatory Potential of Glycoside Flavonoids from Pterocarpus erinaceus Poir. (Fabaceae) Leaves up HPLC Isolation of Rutin, Hesperidin and Quercetin from Ruta Chalepensis Extract Growing in Iraq ›