Activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) Extracts in Capsule Dosage Form to IgM and IgG Levels in Humans

Objective: Traditionally, Pegagan embun (Hydrocotyle sibthorpiodes Lam.) has been used to enhance the immune system. Previous studies confirmed that Hydrocotyle sibthorpiodes Lam. enhanced antibody titers and was practically non-toxic by animal tests. This study aimed to determine the effect of Hydrocotyle sibthorpioides Lam. extract on IgM and IgG levels in humans. Methods: The participants involved 20 healthy volunteers aged 20-25 years were divided into two groups with a placebo as a comparison. The capsules containing Hydrocotyle sibthorpiodes Lam extract were in the treatment group at a dose of 67 mg and the placebo group was given capsules containing lactose orally for three days. IgM and IgG levels were measured before and after consuming the capsules. The ELISA method tested IgM and IgG levels and detected them using specific antibodies that form complexes with enzymes and substrates. Results: The results showed the average baseline IgM level of 1.480 mg/mL and after consumption of 1.405 mg/mL for placebo. For Hydrocotyle sibthorpiodes Lam. extract capsules, the baseline was 1.492 mg/mL and 1.854 mg/mL after consumption. For IgG, before given 8.941 mg/mL and after given 8.710 mg/mL for placebo, while before given Hydrocotyle sibthorpiodes Lam. extract capsules 8.946 mg/mL and after given Hydrocotyle sibthorpiodes Lam. extract capsules was 10.234 mg/mL. Statistical results showed a significant increase in IgM and IgG levels after consuming Hydrocotyle sibthorpiodes Lam. extract capsules for three days (p<0.05). Conclusion: It can be concluded that 67 mg of Hydrocotyle sibthorpiodes Lam. extract capsules can increase IgM and IgG levels in humans.