GC-MS Analysis of Ethyl Acetate Extract of Whole Plant of Rostellularia diffusa

Introduction: To identify the various phyto constituents present in the unexplored plant Rostellularia diffusa (Willd.) by using gas chromatography and mass spectrometry. Methods: The whole plant of Rostellularia diffusa was extracted with ethyl acetate at room temperature for 72 h. The concentrated extract was subjected to GCMS analysis to detect the phyto constituents. Results: Totally 40 compounds were identified and the chromatograph showed 40 peaks with 40 individual compounds. The major constituents were identified in the extract were 16-Hentriacontanone (22.59%), Hexadecanoic acid (11.23%), Stigmast-5-en-3-ol (6.78%), 9-Octadecenoic acid (6.30%) and many other compounds were identified as low level. This preliminary study gives an idea to isolate the major active constituents present in the plant and also helps to develop potential pharmacologically active compounds