ArticleViewAbstractPharmacognosy Journal,2018,10,5,1012-1017.DOI:10.5530/pj.2018.5.172Published:August 2018Type:Original ArticlePhytochemical Test and Cytotoxic Activity of Macroalgae Eucheuma cottonii against Cervical HeLa CellsAde Arsianti, Yully Astika Nugrahayning Aziza, Kartika Dwi Kurniasari, Baiq Kirana Dyahningrum Mandasari, Riathul Masita, Futihati Ruhama Zulfa, Micheylla Kusumaning Dewi, Cut Raisya Zahira Zagloel, Norma Nur Azizah, and Rista Putrianingsih Ade Arsianti1,2,*, Yully Astika Nugrahayning Aziza3, Kartika Dwi Kurniasari3, Baiq Kirana Dyahningrum Mandasari3, Riathul Masita3, Futihati Ruhama Zulfa3, Micheylla Kusumaning Dewi3, Cut Raisya Zahira Zagloel3, Norma Nur Azizah2, Rista Putrianingsih1 1Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA. 2Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA. 3Medical Student, Faculty of Medicine University of Indonesia, Depok, INDONESIA.Abstract:Intoduction: Marine resource of macroalgae Eucheuma cottonii from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. Objectives: Finding cytotoxic activity of Eucheuma cottonii hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. Methods: The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of E. cottonii extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of E. cottonii exhibited a strong cytotoxic activity against cervical HeLa cells with IC50 of 7.54 μg/mL, 5.73 μg /mL, 4.82 μg /mL and 4.34 μg / mL, respectively. Conclusion: The results suggest that macroalgae Eucheuma cottonii could be used as a new anti-cervical cancer’s candidate. Keywords:Anti-cervical cancer, Cytotoxicity, Eucheuma cottonii, HeLa cell lines, Phytochemical test, SeaweedView:PDF (844.2 KB) PDF Images Graphical Abstract ‹ In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells up Effects of Asparagus racemosus Root Extracts on Serum Lipid Profiles, Lipid Peroxidation and Superoxide Dismutase in Ovariectomized Rat ›