ArticleViewAbstractPharmacognosy Journal,2025,17,1,22-27.DOI:10.5530/pj.2025.17.3Published:January 2025Type:Original ArticleAnti-Tyrosinase, Anti-Inflammatory, and Cytotoxic Activity of Si Boo Gan Tang Rice and Rice Bran ExtractsOn-Anong Somsap, Wasinee Poonsawat, Ameena Benchamana, Anussara Kamnate, Gornganok Piboonpol, and Wanita Pantong 1Department of Biochemistry, Faculty of Medicine, Princess of Naradhiwas University, Narathiwat 96000, THAILAND. 2The Center for Scientific and Technological Equipment, Walailak University, NaKhon Si Thammarat 80160, THAILAND. 3Department of Physiology, Faculty of Medicine, Princess of Naradhiwas University, Narathiwat 96000, THAILAND. 4Department of Anatomy, Faculty of Medicine, Princess of Naradhiwas University, Narathiwat 96000, THAILAND. 5Department of Pharmacology, Faculty of Medicine, Princess of Naradhiwas University, Narathiwat 96000, THAILAND. Abstract:Si Boo Gan Tang is a widely cultivated variety of rice in the Tak Bai district of Narathiwat province, located in the southern region of Thailand. This study aimed to evaluate the biological properties of Si Boo Gan Tang rice and rice bran extracts, focusing on their anti-tyrosinase activity, anti-inflammatory activity, and cytotoxicity. The anti-tyrosinase assay revealed that the aqueous extract of rice (RW) significantly inhibited tyrosinase activity by 41.47%. Similarly, the ethanolic extract of rice (RE) exhibited anti-tyrosinase activity at 35.34%. The aqueous (RBW) and ethanolic (RBE) extracts of rice bran showed anti-tyrosinase activity with percentages of 19.26% and 29.08%, respectively. The anti-inflammatory experiment, conducted using RAW 264.7 cells, demonstrated that all extracts (RW, RBW, RE, and RBE) from rice and rice bran exhibited anti-inflammatory properties by reducing the release of nitric oxide (NO) from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The extracts had IC50 values of over 400 μg/ml and CC50 values over 400 μg/ml. Cytotoxicity was assessed using the MTT assay on HaCaT cells. The findings indicated that the IC50 values for both aqueous and ethanolic extracts of rice and rice bran were greater than 800 μg/ml. Keywords:Anti-inflammatory, Anti-tyrosinase, Cytotoxicity, HaCaT cell, Si Boo Gan Tang RiceView:PDF (675.11 KB) PDF Images Cytotoxicity assay of Rice Aqueous Extract (RW) to HaCaT cell. ‹ Marrubium vulgare Leaf Extract-Assisted Green Synthesis of Silver Nanoparticles: Optimization, Characterization and In Vitro Exploration of Their Antimicrobial, Antioxidant and Anti- Inflammatory Potential up Comparison of the Effectiveness of Cream Extracts and VEGF Expression on Incision Wounds in Male Wistar Rats Treated with Ethanol Extracts of Papaya Leaf, Fruit Flesh, and Seeds (Carica Papaya L.) ›