ArticleViewAbstractPharmacognosy Journal,2021,13,5,1269-1276.DOI:10.5530/pj.2021.13.160Published:September 2021Type:Research ArticleIn Vivo Antimammary Tumor Effects of Soybean Extract with Targeted Lunasin (ET-Lun)Numlil Khaira Rusdi, Erni Hernawati Purwaningsih, Andon Hestiantoro, Berna Elya, and Kusmardi Kusmardi Numlil Khaira Rusdi1,2, Erni Hernawati Purwaningsih3,7, Andon Hestiantoro4, Berna Elya5, Kusmardi Kusmardi6-8,* 1Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA. 2Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, INDONESIA. 3Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA. 4Department Obstetrics and Gynaecology, School of Medicine, Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, INDONESIA. 5Department of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, INDONESIA. 6Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA. ' 7Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA. 8Human Cancer Research Cluster, Indonesian Medical Education and Research Institute, Universitas INDONESIA. Abstract:Background/Objective: Lunasin is a peptide, consist of 44 amino acids which have anti-cancer, antioxidant, and anti-inflammatory activity. The price of commercial Lunasin was very expensive due to the high cost of lunasin synthesis and the lack of methods to obtain the pure lunasin weight from plant sources, involving time-consuming analytical instruments. To overcome these problems, the soybean extract with targeted Lunasin (ET-Lun) was made. The aim of this study was to investigate anti-cancer properties of ET-Lun in breast cancer models in vivo. Methods: Effect of ET-Lun was evaluated in 7,12-Dimetilbenz[a]antrasen (DMBA) induced breast cancer rat model. Tumor Mass, volume, and number were measured. The expression of HER2 and EGFR from each treatment group in DMBA-induced rat was evaluated using immunohistochemistry. Results: The results shown that ET-Lun could reduced tumor volume (p=0,021). ET-Lun decreased EGFR expression compared to negative control DMBA (p=0,012). Conclusions: These results indicated that the ET-Lun has anti-breast cancer activity in vivo. Keywords:Breast cancer, EGFR, HER2, In-vivo, SoybeanView:PDF (2.87 MB) PDF Images Graphical Abstract ‹ Amelioration of Cisplatin-Induced Kidney Injury by Pometia pinnata up Determination of Urinary Methyl Hippuric Acid Using Shimadzu UV-Visible Spectrophotometer Among Petroleum Employees ›