Total Phenolic Content and Antioxidant Activity of Different Extracts of Cordia sebestena L. Leaves

Background: Cordia sebestena L. (Boraginaceae) wildly planted in Jakarta, Indonesia. The secondary metabolites of plants have biological and pharmacological activities, such as antioxidant. The antioxidant activity of plants might be due to their phenolic compounds. Objective: To determine the total phenolic content and evaluate the antioxidant activity of C. sebestena leaves extracts (n-hexane, dichloromethane, ethyl acetate, and 70% ethanol). Materials and Methods: The total phenolic content was determined using spectrophotometric methods with a Folin-Ciocalteu reagent. The antioxidant activity of the extracts was determined by its ability to inhibit DPPH radicals through IC₅₀ values (ppm). Results: The highest total phenolic content (167.61 ± 0.56 mg GAE/g) and best antioxidant activity (31.41 ppm) were found in 70% ethanol extract of C. sebestena compared to other extracts (ethyl acetate > dichloromethane > n-hexane). Conclusion: The C. sebestena leaves have a good potential as a source of natural antioxidant, and further research, is recommended to evaluate the antioxidant activity using another method.