Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed- Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10⁹) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.